Louis Pasteur

Louis Pasteur (December 27, 1822 – September 28, 1895) was a French microbiologist and chemist. He is known to the general public for his demonstration of the germ theory of disease and his development techniques of inoculation, most notably the first vaccine against rabies; however, he also made a major discovery in the field of chemistry, regarding asymmetric molecules and the polarization of light.

He also famously quoted: "Le hasard favorise l’esprit preparé" ("Chance favors the prepared mind").


Work on chirality and the polarization of light

In his early work as a chemist he resolved a problem concerning the nature of tartaric acid (1849). A solution of this compound derived from living things (specifically, wine lees) rotated the plane of polarization of light passing through it. The mystery was that tartaric acid derived by chemical synthesis had no such effect, even though its reactions were identical and its elemental composition was the same.

Pasteur noticed, upon examination of the tiny crystals of tartaric acid, that the crystals came in two asymmetric forms that were mirror images of one another. Tediously sorting the crystals by hand gave two forms of tartaric acid: solutions of one form rotated polarised light clockwise, while the other form rotated light counterclockwise. An equal mix of the two had no effect on polarized light. Pasteur correctly deduced that the tartaric acid molecule was asymmetric and could exist in two different forms that resemble one another as a left- and right-hand glove resemble one another. As the first demonstration of chiral molecules, it was quite an achievement, but Pasteur then went on to his more famous work in the field of biology/medicine.

His doctoral thesis on crystallography got him a position of professor of chemistry at the Faculté (College) of Strasbourg.

In 1854, he was named Dean of the new College of Science in Lille. In 1857, he was made administrator and director of scientific studies of the École Normale Supérieure.

Germ theory

Louis Pasteur demonstrated that the fermentation process is caused by the growth of microorganisms, and that the growth of microorganisms in nutrient broths is not due to spontaneous generation.

He exposed boiled broths to air in vessels that contained a filter to prevent all particles from passing through to the growth medium and even in vessels with no filter at all, with air being admitted via a long tortuous tube that would not allow dust particles to pass. Nothing grew in the broths; therefore, the living organisms that grew in such broths came from outside, as spores on dust, rather than being spontaneously generated within the broth. Thus, Pasteur dealt the death blow to the theory of spontaneous generation and supported germ theory.

While Louis Pasteur did not develop germ theory (Girolamo Fracastoro, Friedrich Henle and others had suggested it earlier), he conducted experiments that clearly indicated its correctness and managed to convince most of Europe that it was true.

Pasteur's research also showed that some microorganisms contaminated fermenting beverages. With this established, he invented a process in which liquids such as milk were heated to kill all bacteria and molds already present within them. He and Claude Bernard completed the first test on April 20, 1862. This process was soon afterwards known as pasteurization.

Beverage contamination led Pasteur to conclude that microorganisms infected animals and humans as well. He proposed preventing the entry of microorganisms into the human body, leading Joseph Lister to develop antiseptic methods in surgery.

In 1865, a disease called pebrine was killing great numbers of silkworms. Pasteur worked several years proving that it was a microbe attacking silkworm eggs which caused the disease, and that eliminating this microbe within silkworm nurseries would eradicate the disease.

Pasteur also discovered anaerobiosis - that some microorganisms can develop and live without air or oxygen.

Immunology

His later work on diseases included work on chicken cholera. During this work, a culture of the responsible bacteria had spoiled and failed to induce the disease in some chickens he was infecting with the disease. Upon reusing these healthy chickens, Pasteur discovered that he could not infect them, even with fresh bacteria: the weakened bacteria had caused the chickens to become immune to the disease, although they had not actually caused the disease.

This discovery was an accident. His assistant Charles Chamberland had been instructed to innocuate the chickens after Pasteur went on holiday. Chamberland failed to do this but instead went on holiday himself. On his return the month old cultures made the chickens unwell but instead of the infection being fatal as usual the chickens recovered completely. Chamberland assumed that an error had been made, and wanted to discard the apparently faulty culture out when Pasteur stopped him. Pasteur guessed that the recovered animals now might be immune to the disease as were the animals at Eure-et-Loir that had recovered from anthrax.

In the 1870s he applied this immunization method to anthrax, which affected cattle, and aroused interest in combating other diseases.

Pasteur publicaly claimed that he had made the anthrax vaccine by exposign the bacilus to oxygen. His laboratory notebooks now in the Bibliotheque Nationale in Paris in fact show that Pasteur used the method of a rival Jean-Joseph-Henri Toussaint, a Toulouse veterinary surgeon to create the anthrax vaccine. This method used the oxidizing agent potassium dichromate. Pasteur's oxygen method did eventually produce a vaccine but only after he had been awarded a patent on the production of an anthrax vaccine.

The notion of a weak form of a disease causing immunity to the virulent version was not new: this had been known for a long time for smallpox. Inoculation with smallpox was known to result in far less scarring and greatly reduced mortality than with the naturally acquired disease. Edward Jenner had also discovered vaccination, using cowpox to give cross-immunity to smallpox, and by Pasteur's time this had generally replaced the use of actual smallpox material in inoculation. The difference with chicken cholera and anthrax was that the weakened form of the disease organism had been generated artificially, and so a naturally weak form of the disease organism did not need to be found.

This discovery revolutionised work in infectious diseases, and Pasteur gave these artificially weakened diseases the generic name of vaccines, to honour Jenner's discovery. Pasteur produced the first vaccine for rabies by growing the virus in rabbits and then weakening it by drying the affected nerve tissue.

The rabies vaccine was initially created by Emile Roux, a French doctor and a collegue of Pasteur who had been working with a killed vaccine produced by desiccating the spinal cords of infected rabbits. The vaccine had only been tested in 11 dogs before its first human trial.

This vaccine was first used on 9-year old Joseph Meister on July 6, 1885 after the boy was badly mauled by a rabid dog. This was done at some personal risk for Pasteur, since he was not a licensed physician and could have faced prosecution for treating the boy. Fortunately, the treatment proved to be a spectacular success, with Meister avoiding the disease; thus, Pasteur was hailed as a hero and the legal matter was not pursued. The treatment's success laid the foundations for the manufacture of many other vaccines. The first of the Pasteur Institutes was also built on the basis of this achievement.

Honors and assessment

Pasteur won the Leeuwenhoek medal, microbiology's highest honour, in 1895.

He died in 1895 near Paris from complications caused by a series of strokes that had begun plaguing him as far back as 1868. He was buried in the Cathedral of Notre Dame, but his remains were soon placed in a crypt in the Institut Pasteur, Paris.

Pasteur's method of immunization was effective and was employed by many other physicians, eventually leading to the eradication of the diseases typhus and polio as threats. Pasteurization led to the elimination of contaminated milk and other drinks as sources of disease. In fact, Pasteur inaugurated the modern age of medicine, leading to an increase in the human life span and a surprising population explosion. Accordingly, he has been hailed as the "Father of Medicine" and a "Benefactor of Humanity." Craters on Mars and the Moon are named in his honor. In popular culture, Pasteur is the eponymous French scientist, his name appearing in science fiction shows like Star Trek. A biographical film of his life has also been made, entitled The Story of Louis Pasteur.

Miscellaneous facts

One of the few streets in Saigon,Vietnam that has not been renamed since colonial times is named in honour of Pasteur.


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One of the few streets in Saigon,Vietnam that has not been renamed since colonial times is named in honour of Pasteur. Although Alou's contract includes a player option for 2006, he has stated that he plans to retire if the Giants win the World Series in 2005. A biographical film of his life has also been made, entitled The Story of Louis Pasteur. Because Barry Bonds is already entrenched in left field for the Giants, Alou is expected to move defensively to right field, a position he last played regularly in 2001. In popular culture, Pasteur is the eponymous French scientist, his name appearing in science fiction shows like Star Trek. In December, he signed a one year deal with the Giants worth $13.5 million, with a player option for a second year. Accordingly, he has been hailed as the "Father of Medicine" and a "Benefactor of Humanity." Craters on Mars and the Moon are named in his honor. In October Moises did announce to the public that he had talked to his father, Felipe, about possibly playing for him and the Giants next season.

In fact, Pasteur inaugurated the modern age of medicine, leading to an increase in the human life span and a surprising population explosion. Nevertheless, many experts and reporters doubted the Cubs would pick up his option. Pasteurization led to the elimination of contaminated milk and other drinks as sources of disease. Alou, who was a free agent, said he would love to stay in Chicago. Pasteur's method of immunization was effective and was employed by many other physicians, eventually leading to the eradication of the diseases typhus and polio as threats. However, after high expectations, the Chicago Cubs fell short of a playoff berth when they lost seven of their last nine games. He was buried in the Cathedral of Notre Dame, but his remains were soon placed in a crypt in the Institut Pasteur, Paris. He set new career highs in homeruns (39), doubles (36), and runs (106), while driving in 106 runs.

He died in 1895 near Paris from complications caused by a series of strokes that had begun plaguing him as far back as 1868. After a comeback season in 2003, Alou had a career year in 2004. Pasteur won the Leeuwenhoek medal, microbiology's highest honour, in 1895. The Cubs lost game 7 to the Marlins, who went on to beat the New York Yankees in the World Series. The first of the Pasteur Institutes was also built on the basis of this achievement. The Florida Marlins, Alou's former team, eventually tied the game, took the lead, and won. The treatment's success laid the foundations for the manufacture of many other vaccines. Video replays showed that, although Alou would have had an opportunity to make the catch if Bartman had not reached for the ball, the ball was clearly over the stands, thus fan interference could not be called.

Fortunately, the treatment proved to be a spectacular success, with Meister avoiding the disease; thus, Pasteur was hailed as a hero and the legal matter was not pursued. Alou angrily gestured toward him, but later forgave Bartman. This was done at some personal risk for Pasteur, since he was not a licensed physician and could have faced prosecution for treating the boy. It was the 8th inning of Game 6 of the NLCS, with the Cubs leading and needing only five outs to clinch a World Series birth for the first time since 1945, a Cubs fan named Steve Bartman inadvertantly interfered with a foul ball landing one row into the stands, preventing Alou, who reached into the stands, from catching the ball for an out. This vaccine was first used on 9-year old Joseph Meister on July 6, 1885 after the boy was badly mauled by a rabid dog. In the end, he would make history in the playoffs, but some he would like to forget. The vaccine had only been tested in 11 dogs before its first human trial. Alou lead the team in average in their two series against the Atlanta Braves and Florida Marlins.

The rabies vaccine was initially created by Emile Roux, a French doctor and a collegue of Pasteur who had been working with a killed vaccine produced by desiccating the spinal cords of infected rabbits. However, during the post season, he showed no signs of a slump. Pasteur produced the first vaccine for rabies by growing the virus in rabbits and then weakening it by drying the affected nerve tissue. He ended up with 22 home runs and 91 RBIs. This discovery revolutionised work in infectious diseases, and Pasteur gave these artificially weakened diseases the generic name of vaccines, to honour Jenner's discovery. But a late season slump caused Alou's average to drop to .280. The difference with chicken cholera and anthrax was that the weakened form of the disease organism had been generated artificially, and so a naturally weak form of the disease organism did not need to be found. In the 2003 season, he showed flashes of his old self when he batted over .300 for most of the season while driving in runs as he used to.

Edward Jenner had also discovered vaccination, using cowpox to give cross-immunity to smallpox, and by Pasteur's time this had generally replaced the use of actual smallpox material in inoculation. After the disappointing 2002 season, Alou hired a personal trainer and dedicated himself to return to his old form. Inoculation with smallpox was known to result in far less scarring and greatly reduced mortality than with the naturally acquired disease. He finished up with a disappointing season in his own accounts when he hit only .275 and 15 home runs. The notion of a weak form of a disease causing immunity to the virulent version was not new: this had been known for a long time for smallpox. In 2002, Alou once again ended up on the disabled list at the start of the season, and once healthy, he could never really get into a groove as he did in Houston. Pasteur's oxygen method did eventually produce a vaccine but only after he had been awarded a patent on the production of an anthrax vaccine. In December of 2001, he inked a 3-year, $27 million dollar contract with the Chicago Cubs.

This method used the oxidizing agent potassium dichromate. After the 2001 season, the Astros did not offer Alou a new contract so he in effect became a free agent. His laboratory notebooks now in the Bibliotheque Nationale in Paris in fact show that Pasteur used the method of a rival Jean-Joseph-Henri Toussaint, a Toulouse veterinary surgeon to create the anthrax vaccine. Once recovered, he returned to the Astros lineup to hit .355 and .331 respectively while driving in at least 108 runs in each season. Pasteur publicaly claimed that he had made the anthrax vaccine by exposign the bacilus to oxygen. Alou ended up missing the entire 1999 season. In the 1870s he applied this immunization method to anthrax, which affected cattle, and aroused interest in combating other diseases. However, during the offseason, he would be bitten by the injury bug once more when he tore his ACL in a freak treadmill accident.

Pasteur guessed that the recovered animals now might be immune to the disease as were the animals at Eure-et-Loir that had recovered from anthrax. In his first season with the team, he hit a career high 38 home runs and drove in 124 runs while leading the Astros to a franchise record 102 wins. Chamberland assumed that an error had been made, and wanted to discard the apparently faulty culture out when Pasteur stopped him. In Houston, Alou played the best baseball of his career. On his return the month old cultures made the chickens unwell but instead of the infection being fatal as usual the chickens recovered completely. Before the 1998 season, the Marlins traded Alou to the Houston Astros. Chamberland failed to do this but instead went on holiday himself. In the end, Alou led the team by hitting .321 with three home runs and nine RBIs in the World Series.

His assistant Charles Chamberland had been instructed to innocuate the chickens after Pasteur went on holiday. Florida ended up winning their first World Series in a nail-biting seventh game which ended on an Edgar Rentería base hit. This discovery was an accident. The Marlins made the playoffs as a wildcard team where they defeated first the Giants and then the Atlanta Braves, and advanced to the World Series. Upon reusing these healthy chickens, Pasteur discovered that he could not infect them, even with fresh bacteria: the weakened bacteria had caused the chickens to become immune to the disease, although they had not actually caused the disease. Prior to the 1997 season Alou signed as a free agent with the Florida Marlins, where he led the team with 23 home runs and 115 RBIs. During this work, a culture of the responsible bacteria had spoiled and failed to induce the disease in some chickens he was infecting with the disease. For the next two seasons, he would enjoy stellar seasons at the plate in Montreal, however losing a number of games due to injury.

His later work on diseases included work on chicken cholera. In 1994, he returned to get the game-winning hit in the All-Star Game. Pasteur also discovered anaerobiosis - that some microorganisms can develop and live without air or oxygen. He recovered though, and by 1994 was one of the best hitters in baseball, hitting .339. Pasteur worked several years proving that it was a microbe attacking silkworm eggs which caused the disease, and that eliminating this microbe within silkworm nurseries would eradicate the disease. Alou suffered a severe ankle injury in 1993 that would rob him of his speed and force him to become strictly a corner outfielder. In 1865, a disease called pebrine was killing great numbers of silkworms. In 1990, he was traded to the Montreal Expos where he would later play under his father while he managed the Expos.

He proposed preventing the entry of microorganisms into the human body, leading Joseph Lister to develop antiseptic methods in surgery. In 1986, Alou was the second overall pick in the amateur draft, chosen by the Pittsburgh Pirates. Beverage contamination led Pasteur to conclude that microorganisms infected animals and humans as well. It was there that baseball scouts noticed his tremendous bat speed and speed on the basepaths. This process was soon afterwards known as pasteurization. Alou, who was more interested in playing basketball during his youth, did not play organized baseball until he attended Canada College in California. He and Claude Bernard completed the first test on April 20, 1862. .

With this established, he invented a process in which liquids such as milk were heated to kill all bacteria and molds already present within them. Alou is married to wife Austria Alou; they have three sons: Perico, Kirby and Moisés Jr. Pasteur's research also showed that some microorganisms contaminated fermenting beverages. His father Felipe, the Giants' current manager, as well as uncles Matty and Jesús, all had long and admired careers in the major leagues. While Louis Pasteur did not develop germ theory (Girolamo Fracastoro, Friedrich Henle and others had suggested it earlier), he conducted experiments that clearly indicated its correctness and managed to convince most of Europe that it was true. He comes from a family in which baseball is a way of life. Thus, Pasteur dealt the death blow to the theory of spontaneous generation and supported germ theory. Moisés Rojas Alou (born July 3, 1966 in Atlanta, Georgia) is an All-Star outfielder in Major League Baseball who currently plays for the San Francisco Giants.

Nothing grew in the broths; therefore, the living organisms that grew in such broths came from outside, as spores on dust, rather than being spontaneously generated within the broth. Partially torn calf. He exposed boiled broths to air in vessels that contained a filter to prevent all particles from passing through to the growth medium and even in vessels with no filter at all, with air being admitted via a long tortuous tube that would not allow dust particles to pass. Dislocated shoulder. Louis Pasteur demonstrated that the fermentation process is caused by the growth of microorganisms, and that the growth of microorganisms in nutrient broths is not due to spontaneous generation. Fractured fibula & ankle. In 1857, he was made administrator and director of scientific studies of the École Normale Supérieure. Torn labrum.

In 1854, he was named Dean of the new College of Science in Lille. Torn ACL. His doctoral thesis on crystallography got him a position of professor of chemistry at the Faculté (College) of Strasbourg. Recently, Moisés admitted in an interview with ESPN, that he urinates on his hands frequently to prevent blisters. As the first demonstration of chiral molecules, it was quite an achievement, but Pasteur then went on to his more famous work in the field of biology/medicine. Moisés' best friend and favorite team mate is Jeff Bagwell of the Houston Astros. Pasteur correctly deduced that the tartaric acid molecule was asymmetric and could exist in two different forms that resemble one another as a left- and right-hand glove resemble one another. Many of the horses are named after present and past team mates.

An equal mix of the two had no effect on polarized light. Moisés owns nearly 100 race horses in the Dominican Republic. Tediously sorting the crystals by hand gave two forms of tartaric acid: solutions of one form rotated polarised light clockwise, while the other form rotated light counterclockwise. Alou's parents divorced when he was only a young child. Pasteur noticed, upon examination of the tiny crystals of tartaric acid, that the crystals came in two asymmetric forms that were mirror images of one another. During most of his youth, Moisés lived with his mother. The mystery was that tartaric acid derived by chemical synthesis had no such effect, even though its reactions were identical and its elemental composition was the same. However, father Felipe said young Moisés didn't shed a tear.

A solution of this compound derived from living things (specifically, wine lees) rotated the plane of polarization of light passing through it. As a child, Alou was attacked and nearly killed by a dog. In his early work as a chemist he resolved a problem concerning the nature of tartaric acid (1849). 1998 Silver Slugger Award. . 1994 Silver Slugger Award.
. 1994 NL Comeback Player of the Year.

He also famously quoted: "Le hasard favorise l’esprit preparé" ("Chance favors the prepared mind"). NL All Star 2005. He is known to the general public for his demonstration of the germ theory of disease and his development techniques of inoculation, most notably the first vaccine against rabies; however, he also made a major discovery in the field of chemistry, regarding asymmetric molecules and the polarization of light. NL All Star 2004. Louis Pasteur (December 27, 1822 – September 28, 1895) was a French microbiologist and chemist. NL All Star 2001. NL All Star 1998.

NL All Star 1997. NL All Star 1994.